Two-Day Training on DNA Extraction and EGFR Mutation Analysis at the Cancer Genomics Lab, CApIC-ACE, Covenant University A two-day training workshop on DNA Extraction and EGFR Mutation Analysis was held from 5th to 6th September 2024 at the Cancer Genomics Lab, Covenant Applied Informatics and Communication Africa Centre of Excellence (CApIC-ACE), Covenant University, Ota, Ogun State. The workshop, a collaborative initiative between the National Institute for Cancer Research and Treatment (NICRAT) and AstraZeneca Nigeria, aimed to equip participants with advanced diagnostic techniques for lung cancer, specifically non-small cell lung cancer (NSCLC). The primary goal was to enhance participants' understanding of DNA extraction and EGFR mutation analysis—key aspects of precision medicine in cancer treatment—and to train personnel for precision medicine initiatives between CApIC-ACE, NICRAT, and AstraZeneca
Day 1: Theoretical Foundations
The workshop commenced with a series of theoretical sessions designed to provide participants with a strong foundation in the principles of DNA extraction and its practical applications in molecular diagnostics. The discussions covered various DNA isolation techniques from clinical samples, emphasizing critical steps to ensure high-quality DNA for subsequent mutation analysis. Facilitators delivered comprehensive presentations on EGFR mutations, explaining their role in the development and progression of lung cancer. This theoretical framework provided essential context for the practical sessions scheduled for the second day.
Day 2: Practical Training and Hands-on Application
The second day was devoted entirely to hands-on practical training, where participants applied the theoretical knowledge gained from the previous day. Under the supervision of experienced facilitators, participants performed DNA isolation from clinical samples and conducted EGFR mutation assays using specialized assay kits. Step-by-step demonstrations allowed participants to master DNA extraction, mutation analysis, and interpretation of EGFR mutation results. The practical sessions underscored the clinical implications of identifying EGFR mutations in cancer patients, particularly in guiding the selection of targeted therapies for NSCLC. This highlighted the transformative potential of personalized medicine in improving patient outcomes.
Workshop Outcomes
The workshop successfully achieved its objectives by integrating theoretical learning with practical application. Participants acquired the skills and confidence needed to independently perform DNA extraction and EGFR mutation analysis using advanced diagnostic tools. Moreover, they gained a deeper understanding of the clinical relevance of EGFR mutation testing in advancing targeted cancer therapies.
Collaboration and Impact
The workshop underscored the successful collaboration between NICRAT, AstraZeneca Nigeria, and CApIC-ACE, emphasizing the importance of partnerships in enhancing cancer research and diagnostic capabilities in Nigeria. This training is expected to have a long-term impact on improving early detection and personalized treatment for lung cancer, thereby addressing a significant public health issue in the region.
